Efficient magnesium-dependent human immunodeficiency virus type 1 integrase activity

Functional interactions of human immunodeficiency virus type 1 integrase with human and yeast HSP60.

Integration of human immunodeficiency virus type 1 (HIV-1) proviral DNA in the nuclear genome is catalyzed by the retroviral integrase (IN). In addition to IN, viral and cellular proteins associated in the high-molecular-weight preintegration complex have been suggested to be involved in this process. In an attempt to define host factors interacting with IN, we used an in vitro system to identi...

Resistance mechanism of human immunodeficiency virus type-1 protease to inhibitors: A molecular dynamic approach

Human immunodeficiency virus type 1 (HIV-1) protease inhibitors comprise an important class of drugs used in HIV treatments. However, mutations of protease genes accelerated by low fidelity of reverse transcriptase yield drug resistant mutants of reduced affinities for the inhibitors. This problem is considered to be a serious barrier against HIV treatment for the foreseeable future. In this st...

Identification and characterization of persistent intracellular human 1 immunodeficiency virus type 1 integrase strand transfer inhibitor activity 2 3

15 16 17 Correspondent footnote: 18 Dr. Alan Engelman, Department of Cancer Immunology and AIDS, Dana-Farber Cancer 19 Institute, 44 Binney Street, CLS-1010, Boston, MA 02115. Phone: (617) 632-4361. Fax: (617) 20 632-3113. E-mail: [email protected]. 21 Copyright © 2010, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. Antimicrob. Agents...

Quantification Analysis of Dot Blot Assays for Human Immunodeficiency Virus Type 1 and 2 Antibodies

Objective Dot Blot (DB) assay provides highly specific results, but usually not reliable for quantification of antibody production. The need for a more objective DB assay to provide a better definition of the immune status, against HIV antigens, promoted this study to be done to develop a quantitative DB assay. Material and Methods Dot blot (DB) strips for antibodies directed to human immuno...

Phagocytic activity in human immunodeficiency virus type 1 infection.